Morris water maze behavioral and functional neuroscience. Pdf cognitive evaluation using morris water maze in. Screen capture from the anymaze video tracking system demonstrating pool calibration. The morris water maze test is a behavioral task used to study spatial learning. Morris water maze test an overview sciencedirect topics. Morris in 1981 in order to test hippocampaldependent learning, including acquisition of spatial memoryand longterm spatial memory 1.
The animaltracker is a universal tracking application specifically designed to support animal behavioral analyses. The morris water maze task is a widely used tool to assess hippocampusdependent. Morris water maze training in opaque water 4 duration. This is an open source project, based on the imagej image processing program. Obtain these measurements from the water maze tab under experimental list and export directly to an excel spreadsheet.
Tempera paint is added into the water until it becomes opaque. The morris water maze mwm is a test of spatial learning for rodents that relies on distal cues to navigate from start locations around the perimeter of an open swimming arena to locate a. The mwm is a relatively simple procedure typically consisting of six day trials, the main advantage being the differentiation between the spatial hiddenplatform and nonspatial. The procedure described here contains two critical variables which represent a deviation from other versions of the water maze. Morris water maze test for learning and memory deficits in. Circular pools for morris water maze test ugo basile. Automate your test easily with our video tracking system and high quality mazes.
The morris water maze mwm was first established by neuroscientist richard g. The morris water maze is a behavioral task to test. The morris water maze is a wellaccepted tool used to document the. Ethovision xt, the most widely applied video tracking software in animal behavioral research, is ideal for the automated tracking and analysis of animal movement in a morris water maze. This task can be altered in numerous ways to investigate working memory, reference memory and task strategy 6.
Pdf morris water maze is a behavioral test used to study spatial learning and memory in experimentals. Ethovision xt is the most widely applied video tracking software that tracks and analyzes the behavior, movement, and activity of any animal. The use of a hand timer can also be beneficial to ensure the accuracy of the tracking software. The morris water maze tests impairments in visual shortterm memory and visual spatial abilities in small rodents 69, 70. If you are recording the task with a video camera, make sure all sides of the maze are within the cameras field of view. Autoplay when autoplay is enabled, a suggested video will. A costeffective solution for all standard behavioral tests such as the morris water maze and open field testing. Transgenic mouse models have been instrumental in evaluating the pathophysiology of. The morris water maze mwm is a test of spatial learning for rodents that relies on distal cues to navigate from start locations around the. Pdf video analysis of morris water maze experiments. Copy citation download citations reprints and permissions. The morris water maze mwm is designed to test spatial memory and long term memory by observing and recording escape latency, thigmotaxis duration, distance moved, and velocity during the time spend in the mwm water tank.
Swimming activity of the rats was monitored via a video camera mounted. You can also download the free morris water maze white paper. Ethovision xt, the most widely applied video tracking software in animal. Video analysis of morris water maze experiments researchgate. This is a wild type mouse showing spatial learning over a 5 day period in a morris water maze. Our water mazes are 60cm high from bottom floor to edge, and are available in the following standard diameters.
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